Category Archives: Cannabinoid Receptor

Stage-Specific Excitation of Cannabinoid Receptor: DISCUSSION(3)

Since COX-2, but not COX-1, is capable of converting anandamide to PGE2 ethanolamide, it is possible that the relatively lower levels of anandamide at the implantation sites compared with higher levels at the interimplantation sites are regulated by COX-2. COX-2 … Continue reading

Stage-Specific Excitation of Cannabinoid Receptor: DISCUSSION(2)

The differentiation-promoting effects of cannabinoids on trophoblast cells at low nanomolar levels are surprising, since similar concentrations were inhibitory at the earlier stages of embryonic development. This stage-specific effect may explain why the preimplantation uterine environment is normally hostile to … Continue reading

Stage-Specific Excitation of Cannabinoid Receptor: DISCUSSION(1)

The ‘‘window’’ for successful implantation is defined as the limited time span during which the activated stage of the blastocyst is superimposed on the receptive state of the uterus. The molecular signaling that determines this window of implantation is not … Continue reading

Stage-Specific Excitation of Cannabinoid Receptor: RESULTS(4)

Again, the application of CB1-R antagonist blocked the acceleration of trophoblast differentiation induced by canna-binoids (Table 1). These observations, consistent with our FBA experiments, strongly indicated that cannabinoids at a concentration of 7 nM effectively accelerated periimplan-tation trophoblast differentiation. To … Continue reading

Stage-Specific Excitation of Cannabinoid Receptor: RESULTS(3)

These results establish that the activation of CB1-R, but not CB2-R, stimulates trophoblast cell differentiation. The treatment of blastocysts with either of the antagonists alone did not influence their differentiation (Fig. 2). However, FBA of blastocysts treated with SR141716A appeared … Continue reading

Stage-Specific Excitation of Cannabinoid Receptor: RESULTS(2)

Cannabinoid-treated embryos exhibited down-regulation of FBA at 72 h, consistent with their accelerated rate of differentiation. Each of the three agonists shifted the developmental profile by approximately 24 h as compared to the vehicle treatment (p < 0.01), demonstrating that … Continue reading

Stage-Specific Excitation of Cannabinoid Receptor: RESULTS(1)

Cannabinoids Promoted Trophoblast Differentiation As previously reported, FBA is developmentally regulated during serum-free culture of blastocysts. The normal developmental profile is distinguished by low levels of FBA after 48 h, high levels at 72 h, and low levels again after … Continue reading

Stage-Specific Excitation of Cannabinoid Receptor: MATERIALS AND METHODS(3)

The binding assay was conducted by incubating embryos at 4°C for 30 min in a 0.2% suspension of FN-120-coated microspheres prepared in PBS/BSA. Blastocysts were then washed free of unbound beads by transfer through four drops of PBS/BSA and fixed … Continue reading

Stage-Specific Excitation of Cannabinoid Receptor: MATERIALS AND METHODS(2)

In some experiments, embryos were washed free of cannabinoids or vehicle after 30 min of incubation and then cultured without further addition of the ligands. For reversal experiments, blastocysts were cultured for 1 h with 8 nM SR141716A, a CB1-R … Continue reading

Stage-Specific Excitation of Cannabinoid Receptor: MATERIALS AND METHODS(1)

Production and Culture of Embryos B6CBAF1/J mice (Jackson Laboratory, Bar Harbor, ME) were housed in the C.S. Mott Center vivarium at Wayne State University according to National Institutes of Health and institutional guidelines on the care and use of laboratory … Continue reading