Category Archives: Clonal Sertoli Cell

Characterization of a Clonal Sertoli Cell Line: DISCUSSION(6)

This cell shape, which has been previously reported in FSH-stimulated primary culture of Sertoli cells, is specific to this gonadotropin and was not observed with hCG. The possibility that this change in Sertoli cellular architecture is associated with specific cellular … Continue reading

Characterization of a Clonal Sertoli Cell Line: DISCUSSION(5)

Sertoli cell . Since the immortalized cells are negative for 3(3-hydroxysteroid dehydrogenase and lactate dehydro-genase-X (data not shown), specific markers of Leydig cells and of meiotic and postmeiotic germ cells, respectively, it is likely that the 42GPA9 cell line is … Continue reading

Characterization of a Clonal Sertoli Cell Line: DISCUSSION(4)

The present data agree with the recent observation that tissue-type PA is the main PA activity produced by the mouseĀ of the tight junction-associated protein ZO-1 have been reported during development . However, the molecular and cellular events that trigger formation, … Continue reading

Characterization of a Clonal Sertoli Cell Line: DISCUSSION(3)

Thus, a possible explanation is that the transgene insertion induces genomic events that promote and maintain cell growth in the 42GPA9 cell line independently of germ cell control.

Characterization of a Clonal Sertoli Cell Line: DISCUSSION(2)

The reason for the discrepancy between the previous findings and the current study is presently unclear. Although the same strategy was used to generate PyLT transgenic mice, the two immortalized cell lines originate from two different families, and differences between … Continue reading

Characterization of a Clonal Sertoli Cell Line: DISCUSSION(1)

The establishment of a well-characterized Sertoli cell line was a prerequisite for subsequent in vitro analysis of the physiological role of this cell type in germ cell proliferation and differentiation. Targeted oncogenesis in transgenic mice had succeeded in producing testicular … Continue reading

Characterization of a Clonal Sertoli Cell Line: RESULTS(6)

In addition, the reaction was negative in the absence of reverse transcriptase in the cell line and testicular samples. The possibility of cross-tissue contamination could be excluded since no RT-PCR product was detected when primers were used with RNA isolated … Continue reading

Characterization of a Clonal Sertoli Cell Line: RESULTS(5)

Poly (A)+ RNAs isolated from the 42GPA9 cell line, and testes from an adult B6D2 animal and from an adult PyLT transgenic mouse, were subjected to RT-PCR. Visualization of the products by ethidium bromide staining revealed four identical major bands … Continue reading

Characterization of a Clonal Sertoli Cell Line: RESULTS(4)

FSH Responsiveness of the 42GPA9 Cell Line and Expression of FSH Receptor The 42GPA9 cell line underwent morphological changes when cultured in the presence of 100 mlU/ml recombinant human FSH or oFSH. Similar results were obtained with the two hormones. … Continue reading

Characterization of a Clonal Sertoli Cell Line: RESULTS(3)

KL. Proteins from 42GPA9 cell lysates were analyzed by SDS-PAGE (12%). Western blot analysis for KL revealed the presence of an immunoreactive band of approximately 28 kDa that may correspond to the presumed cell membrane form of KL-2 . This … Continue reading