Category Archives: Embryos

Effects of Male Accessory Sex Glands: DISCUSSION(4)

The present study shows that the S-phase of the first cell cycle in the golden hamster begins at about 6 h. p.c. and lasts for about 5-6 h. This is different from that observed by Naish et al.. In our … Continue reading

Effects of Male Accessory Sex Glands: DISCUSSION(3)

Our results also suggest that the mechanism of action of AG and VP may be different. In the AGX group pulse-labeled for 30 min in BrdU, the proportion of embryos with one or two pronuclei in S-phase at 8 h … Continue reading

Effects of Male Accessory Sex Glands: DISCUSSION(2)

The main contribution of a sperm to the first cell cycle is to trigger Ca2+ transients, which are believed to destroy cytostatic factor and thence lead to the loss of maturation-promoting factor (MPF) activity. Inactivation of MPF in turn influences … Continue reading

Effects of Male Accessory Sex Glands: DISCUSSION(1)

Genetic factors have long been known to affect male fertility by influencing the timing of activation of the oocyte and the formation of the pronuclei at fertilization. The extent to which nongenetic factors affect male fertility is controversial. Our previous … Continue reading

Effects of Male Accessory Sex Glands: RESULTS(2)

When embryos were collected at 6 h p.c. and cultured in TALP containing 50 p.M BrdU for 6 h, no significant difference was observed in terms of percentage of embryos with one or two pronuclei in S-phase in the four … Continue reading

Effects of Male Accessory Sex Glands: RESULTS(1)

Effects of ASG, AG, or VP on Fertilization Our previous study showed that sperm penetration of the zona pellucida began at around 4 h p.c. in the hamster. In this study, we found that at 6 h p.c. more than … Continue reading

Effects of Male Accessory Sex Glands: MATERIALS AND METHODS(3)

The slides were washed with PBS and incubated for 1 h with 50 |xl fluorescein-labeled sheep-anti mouse IgG at a 1:100 dilution in PBS containing 1 (Ag/ml BSA. The oocytes/zygotes were examined with an epiflu-orescent microscope equipped with a 450-490-nm excitation … Continue reading

Effects of Male Accessory Sex Glands: MATERIALS AND METHODS(2)

Collection of Oocytes/Zygotes Female hamsters were killed by overdose of inhaled chloroform at 6, 8, 10, and 12 h p.c. Eight females were used for each experimental group. Oocytes/zygotes were flushed from the oviducts with TALP medium equilibrated with 5% … Continue reading

Effects of Male Accessory Sex Glands: MATERIALS AND METHODS(1)

Animals Randomly bred golden hamsters (Mesocricetus auratus) were fed with standard food and tap water ad libitum and maintained in the Laboratory Animal Unit of the Faculty of Medicine, The University of Hong Kong, under a 14L: 10D regime (lights-on … Continue reading

Effects of Male Accessory Sex Glands on Deoxyribonucleic Acid Synthesis in the First Cell Cycle of Golden Hamster Embryos(2)

In most mammals, fertilization occurs at the metaphase of the second meiotic division when an oocyte is penetrated by a sperm. The transformation of male and female nuclei into fully developed male and female pronuclei involves a complicated series of … Continue reading