Category Archives: HIV

Expression of Surface Markers on Alveolar Macrophages From Symptomatic Patients with HIV Infection as Detected by Flow Cytometry: Comment

Although DNA amplification has latent infection in 47 to 100 percent of AM samples taken from patients with AIDS, the number of infected AMs in each sample may also be very low due to the high sensitivity of the polymerase … Continue reading

Expression of Surface Markers on Alveolar Macrophages From Symptomatic Patients with HIV Infection as Detected by Flow Cytometry: Conclusion

The results indicate an upregulation of HLA-DP, CDllb, CDllc, CD14, and CD33. The increased expression of CDllb, CD14, and CD33, typical monocyte markers, suggests increased monocyte recruitment. To test this hypothesis, we analyzed the expression of CD14, CDllb, HLA class … Continue reading

Expression of Surface Markers on Alveolar Macrophages From Symptomatic Patients with HIV Infection as Detected by Flow Cytometry: Discussion

Difficulties in interpreting data on marker expression are often methodologic. Results obtained by immunocytochemistry cannot be directly compared with those obtained by flow cytometry. Immunocytochemistry does not permit quantification of marker expression, although it offers the advantage of direct visualization … Continue reading

Expression of Surface Markers on Alveolar Macrophages From Symptomatic Patients with HIV Infection as Detected by Flow Cytometry: Cellular Composition of the BAL

In agreement with previous reports, absolute cell counts, absolute and percent total lymphocytes, and absolute and percent suppressor/cytotoxic T cells were increased significantly in the BAL of HIV-infected patients (Table 5). There was a concomitant decline in percent macrophages, CD4 … Continue reading

Expression of Surface Markers on Alveolar Macrophages From Symptomatic Patients with HIV Infection as Detected by Flow Cytometry: Results

The use of prophylactic agents has been postulated to modify local pulmonary defenses in HIV-infected patients. Therefore, we analyzed our data for a possible effect of current pentamidine inhalation and of zidovudine (AZT). Regular pentamidine prophylaxis was associated with a … Continue reading

Expression of Surface Markers on Alveolar Macrophages From Symptomatic Patients with HIV Infection as Detected by Flow Cytometry: Results

The expression of HLA-DP was enhanced substantially on AMs of HIV-infected patients (12.1 ± 1.5 vs 6.5 ±0.9 for controls, p=0.01) (Table 3 and Fig 1). Neither HLA-DR, which had the highest relative surface density among all markers studied (36.9 … Continue reading

Expression of Surface Markers on Alveolar Macrophages From Symptomatic Patients with HIV Infection as Detected by Flow Cytometry: Statistics

Log green fluorescence intensity of 10,000 cells was displayed on a histogram in arbitrary units. From given median channel number, the data were transformed to calculate the linear median intensity of the entire AM population. To obtain a value for … Continue reading

Expression of Surface Markers on Alveolar Macrophages From Symptomatic Patients with HIV Infection as Detected by Flow Cytometry: Surface Marker Analysis

Phenotyping was carried out immediately after BAL fluid collection. For security reasons, BAL fluid from HIV-positive patients was labeled clearly as such. After centrifugation for 10 min at 400 g, cells were washed twice in minimal essential medium (MEM) (Life … Continue reading

Expression of Surface Markers on Alveolar Macrophages From Symptomatic Patients with HIV Infection as Detected by Flow Cytometry: Methods

Patients and Controls Bronchoalveolar lavage (BAL) fluid was obtained from a total of 32 consecutive nonselected HIV-infected patients presenting with respiratory symptoms (cough or mild dyspnea or both). Bronchoscopy, including lavage and transbronchial biopsy, was indicated to obtain a diagnosis … Continue reading

Expression of Surface Markers on Alveolar Macrophages From Symptomatic Patients with HIV Infection as Detected by Flow Cytometry

Deficient monocyte/macrophage function has been suggested to be a leading cause of opportunistic disease in HIV-infected patients. However, evidence to support this idea is far from conclusive. Most investigations have been carried out on peripheral blood monocytes (PBMs). In these … Continue reading