Category Archives: Oocytes

Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release: DISCUSSION(7)

Research on mammalian spermatozoa has shown the presence of a detergent-insoluble complex that is specifically adherent to the luminal surface of the outer acrosomal membrane in cows and hamsters. In 1996, NagDas et al. termed this detergent-insoluble continuous sheet attached … Continue reading

Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release: DISCUSSION(6)

The requirement for 6-DMAP in the activation protocol may suggest a possible mechanism for sperm-egg binding and subsequent activation. The ability of the peptide to initiate intracellular Ca2+ transients but not full activation as evidenced by parthenogenetic development may suggest … Continue reading

Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release: DISCUSSION(5)

At all concentrations of ryanodine evaluated, the germinal vesicle (GV) oocytes either failed to respond or exhibited a reduced intracellular calcium response as compared to metaphase II (MII) oocytes. Similar results were reported by Yue et al. when comparing the … Continue reading

Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release: DISCUSSION(4)

The third hypothesis proposes that the sperm acts as a channel or pore to allow extracellular Ca2+ to enter the egg causing intracellular Ca2+ release. Alternatively, in 1983 Jaffe suggested that the sperm may be the source of Ca2+; favored … Continue reading

Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release: DISCUSSION(3)

Three different hypotheses have been put forth to explain how sperm initiate [Ca2+]i oscillations in mammalian eggs. One hypothesis is that spermatozoa interact with a receptor located in the plasma membrane of the oocyte. This receptor is postulated to be … Continue reading

Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release: DISCUSSION(2)

Although an RGD-containing peptide accompanied [Ca2+]i release in Xenopus, this is the first published report in mammalian species that demonstrates the ability of an RGD-containing peptide to initiate intracellular Ca2+ transients similar to those observed at fertilization. The ability of … Continue reading

Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release: DISCUSSION(1)

In this study, we reveal a potential role for integrins in sperm-egg interactions by investigating the effects of an RGD-containing peptide on in vitro fertilization, generation of intracellular Ca2+ transients, and parthenogenetic activation and subsequent development of bovine oocytes. In … Continue reading

Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release: RESULTS(2)

Ability of an RGD-Containing Peptide to Induce Intracellular Calcium Transients To establish whether an RGD-containing peptide could induce intracellular Ca2+ transients similar to those observed at fertilization, MII bovine oocytes were incubated in the presence of the RGD- or non-RGD-containing … Continue reading

Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release: RESULTS(1)

Ability of an RGD-Containing Peptide to Bind to the Plasma Membrane of Bovine Oocytes After removal of the ZP, bovine oocytes were able to recognize an RGD-containing peptide in that fluorescent immunobeads coated with GRGDSPK (RGD-containing peptide) bound to the … Continue reading

Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release: MATERIALS AND METHODS(4)

Intracellular Calcium Monitoring and Calibration Immediately prior to calcium measurements, the Fura-2 loaded oocytes were transferred to a 30-^l drop of M2-medium containing either 1 mg/ml of non-RGD or 1 mg/ ml of RGD peptide and covered with warmed mineral … Continue reading