Category Archives: Plasminogen

Human Endothelial Cell Migration: DISCUSSION(6)

We found no effects of free or complexed uPA on thymidine incorporation in the endothelial cells. Such effects, however, may be dependent on tissue culture conditions, since it has been shown that the response of endothelial cells to stimulants like … Continue reading

Human Endothelial Cell Migration: DISCUSSION(5)

Since the amount of TGFp1 mRNA is highest in late secretory and menstrual endometrium, and high PA activity, which can initiate activation of latent TGFps, is released premenstrually, we have suggested that TGFpl participates in the postmenstrual repair process. The … Continue reading

Human Endothelial Cell Migration: DISCUSSION(4)

There is experimental evidence to indicate that endometrial angiogenesis is regulated by endometrial cells, i.e., endometrial explants as well as separated stromal and epithelial cells influence vascularization in a modified chorioallantoic membrane assay, and cocultures of endothelial cells with endometrial … Continue reading

Human Endothelial Cell Migration: DISCUSSION(3)

The stimulatory curves were almost identical, which agrees with previous observations that the active site of uPA is not needed for the binding to the receptor nor for initiating signal transduction and cellular effects. On the other hand, monoclonal antibodies … Continue reading

Human Endothelial Cell Migration: DISCUSSION(2)

The binding of uPA to its receptor has been shown to induce intracellular phosphorylation processes involving protein tyrosine kinase. Cellular effects that follow the binding of uPA or its amino-terminal fragment to the receptor include proto-oncogene expression, mitogenic effects, cell … Continue reading

Human Endothelial Cell Migration: DISCUSSION(1)

Our results show that endometrial stromal cells in culture stimulated with TGFp1 increase their extracellular levels of uPA, PAI1, and uPA:PAI1 complex. The increase of PAI1 is a transcriptional effect, since we have demonstrated that stimulation with TGFp1 of stromal … Continue reading

Human Endothelial Cell Migration: RESULTS(3)

The above results imply a possible paracrine regulatory loop involving the release by TGFp1-stimulated endometrial stromal cells of uPA:PAI1 complex, which subsequently stimulates migration in adjacent endothelial cells. We also considered an alternative model for stromal stimulation of endothelial cell … Continue reading

Human Endothelial Cell Migration: RESULTS(2)

These two cell-types represent both large-vein endothelial cells and microvascular endothelial cells (Figs. 4 and 5). Stimulation by both uPA and the complex was maximal at 1 nM. Similarities between the curves for free and complexed uPA support the conclusion … Continue reading

Human Endothelial Cell Migration: RESULTS(1)

Endometrial stromal cell cultures treated with TGFp1 contained increased conditioned medium concentrations of uPA and PAI1 (Fig. 1). Consequently, the concentration of the uPA:PAI1 complex was also increased in the medium. Elimination of complexed uPA, i.e., internalization for lysosomal degradation, … Continue reading

Human Endothelial Cell Migration: MATERIALS AND METHODS(8)

After this time, the cells were fixed with absolute methanol for 10 min and subsequently stained with Giemsa (12.5%)¬†for 15 min. The number of cells that had migrated across the wound line were counted per high-power field. The mean of … Continue reading