Category Archives: Transcription Factor

Defective Induction of the Transcription Factor Interferon: DISCUSSION(7)

As described above, however, we have not found an activity with similar inhibitory properties in JEG-3 cells. Therefore, the precise mechanism interfering with p48/ISGF37 expression and/or activity remains elusive. antibiotic levaquin The screening of cell lines for mutations in IFN … Continue reading

Defective Induction of the Transcription Factor Interferon: DISCUSSION(6)

It was surprising, though, that while IFN7 was able to increase p48/ISGF37 in JEG-3 cytoplasmic extracts to levels similar to HeLa cells, this still did not restore ISGF3-inducibility. This represents a significant difference between JEG-3 and HeLa cells. It seems … Continue reading

Defective Induction of the Transcription Factor Interferon: DISCUSSION(5)

Our finding that JEG-3 cells could be protected by IFN against EMCV is similar to results in BeWo and HCCM-5 trophoblast cells and suggests that IRF-1-dependent pathways are normally stimulated in trophoblast cells. In support of this, IFNa induction of … Continue reading

Defective Induction of the Transcription Factor Interferon: DISCUSSION(4)

The HCCM-5 and BeWo trophoblast cell lines were also reported to be refractory to the antiviral effect of IFNp towards VSV. Type I IFNs (a, p, w) activate several independent signal transduction pathways downstream of a common receptor. Activation of … Continue reading

Defective Induction of the Transcription Factor Interferon: DISCUSSION(3)

Nonetheless, in our experiments, overall MHC class I expression was unaffected by IFN (including IFNa and IFN7), indicating that the response of JEG-3 cells to IFN7 is insufficient to induce HLA-A, -B, and -C genes. This conclusion is supported by … Continue reading

Defective Induction of the Transcription Factor Interferon: DISCUSSION(2)

The overall significance of changes in gene expression for trophoblast cell function is unclear at present. However, we suggest that this defect in IFN signal transduction may be one mechanism, perhaps among others, for preventing the expression of the classical … Continue reading

Defective Induction of the Transcription Factor Interferon: DISCUSSION(1)

In this study, we found that human trophoblast cells show diminished responses to IFNa including the inability of IFN to induce MHC class I expression. One surprising explanation for this finding is that IFNa signal transduction, which normally culminates via … Continue reading

Defective Induction of the Transcription Factor Interferon: RESULTS(8)

ISGF3y Was Limiting to ISGF3 Complex Formation Low levels of p48/ISGF37 protein and binding activity in the nucleus of control and IFN-treated JEG-3 cells suggested that it alone may limit formation of ISGF3. To test this idea, we took advantage … Continue reading

Defective Induction of the Transcription Factor Interferon: RESULTS(7)

IFN7 treatment for 16 h was associated with a significant increase in total cellular content of p48/ISGF37 protein in JEG-3 cells, similar to that in HeLa cells (Fig. 6B). Im-munostaining of JEG-3 cells demonstrated that p48/ISGF3^ was up-regulated after IFN7 … Continue reading

Defective Induction of the Transcription Factor Interferon: RESULTS(6)

Low Levels of p48/ISGF3y Were Detectable in JEG-3 Nuclear Extracts Because STAT1 and STAT2 appeared to be properly activated by IFNa in JEG-3 cells, one possible mechanism for the failure to induce ISGF3 would be reduced expression of the p48/ISGF37 … Continue reading