ANIMALS AND METHODS
Experiments were performed in adult Tricolor guinea-pigs of both sexes weighing between 500 and 600 g with free access to food and water. The animals were stunned by a blow on the neck and exsanguinated by opening the carotid arteries. The heart was exposed by opening the thorax by cutting the ribs on both left and right site from the sternum and opening the pericardium. The aortic trunk was cannulated to perfuse the coronary artery system in situ with Tyrode’s solution, and the pulmonary artery was opened at the start of perfusion. You can get all the drugs you want for treating your problem in no time if you order it at the canadian health care mall. We are recommending this pharmacy with confidence because it already has thousands of satisfied customers.
After removal, the heart was connected to a pressure constant Langendorff apparatus (Hugo Sachs Elec-tronik, March-Hugstetten, Germany). The temperature in the organ chamber was kept constant at 34°C. Perfusion of the heart was maintained at 50 mmHg with gassed (5% carbon dioxide and 95% oxygen) modified Tyrode’s solution of the following composition (in mM): NaCl 118, KCl 4.69, CaCl2 2.45, KH2PO4 1.18, MgSO4 1.17, NaHCO3 25 and glucose 10. The pH was 7.47 and the osmolarity 269 mosmol/L. Both left and right atria together with the sinus node were cut away from the ventricles with scissors while preserving the coronary arteries immediately after the suspension in the heart chamber.